Tecan Safire (Tecan) is a UV-Vis fluorescence and absorbance plate reader providing the high throughput and accuracy ideal for use in modern drug discovery and research. This microplate reader has high sensitivity, excellent performance and built in flexibility, in a compact format, that allows users to quickly change applications without cumbersome filter changes. With its fast wavelength scanning and high sensitivity, users get results in short measurement times.
Method of quantification :
PicoGreen dsDNA quantitation: selective quantification of dsDNA with a fluorescent DNA-binding dye from 0.2 to 100 ng/µl, with only 1 µl of DNA. This dye shows minimal binding to ssDNA and RNA, allowing specific quantitation of dsDNA.
RiboGreen RNA quantitation: selective quantification of ssRNA with a fluorescent RNA-binding dye from 1 to 500 ng/µl, with only 1 µl of RNA.