Qubit® 2.0 Fluorometer (ThermoFisher Scientific) is a benchtop fluorometer for DNA, RNA and protein quantitation using the highly sensitive and accurate fluorescence-based specific probes.
Method of quantification :
PicoGreen dsDNA quantitation: selective quantification of dsDNA with a fluorescent DNA-binding dye from 0.2 to 100 ng/µl, with only 1 µl of DNA. This dye shows minimal binding to ssDNA and RNA, allowing specific quantitation of dsDNA.
RiboGreen RNA quantitation: selective quantification of ssRNA with a fluorescent RNA-binding dye from 1 to 500 ng/µl, with only 1 µl of RNA.