DNA resequencing starts with gDNA from many source of interest: eukaryote, prokaryote, virus, BAC, fosmids, plasmids, etc, which have already a reference genome for comparison. The gDNA is fragmented and sequenced to generate sequencing reads (fastQ). Then this reads are aligned and assembled on the reference genome. The procedure is used to detect mutations (SNP, InDel…) compared to the reference genome.
Whole Genome Sequencing
DNA Resequencing
DNA de novo sequencing
DNA de novo sequencing refers to sequence a novel genome where no reference genome is available. It starts with gDNA from many source of interest: eukaryote, prokaryote, virus, BAC, fosmids, plasmids, etc. The gDNA is fragmented and sequenced to generate sequencing reads (fastQ). This reads are then assembled into contigs and scaffolds to reconstruct the genome as complete as possible.
How to use our Whole Genome Sequencing by NGS services ?
After discussion with our team, we offer a personalized solution to your project, and tailor-made according to your goals and depending on your genome source.
After DNA-seq librairies constructions, your gDNA will be sequenced on a paired-end 2×300 bp run or other to generate up to 25M of reads on our MiSeq system for small genomes, and on a paired-end 2×150 bp run or other on NextSeq for eukayote genomes. Then, our bioinformatic solutions will then process the datas, to align the sequences on a reference genome to detect variants, or to assemble your genome as complete as possible.
You can trust on our reactivity, fast delivery, low prices and scientific support for the best adaptation of protocols and techniques to the goals of your projects.
Your publications
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Hugouvieux-Cotte-Pattat, N et al. J Genomics. 2019; 7: 64–72
Normand P et al. Int J Syst Evol Microbiol. 2018 Jul 30 Biofidal has the pleasure to be co-author on this paper
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