• High quality sequence: Read length up to 1 kb with a Q20 on ABI 3730XL equipment.
• Rapid turnaround time: Within 24 hours after sample receipt.
• Flexible sample submission and pickup: samples submitted in different tubes or 8well-strips.
• Wide range of sample: plasmids, cosmids/fosmids/BAC, PCR products, single-strand DNA.
• Free services: Free PCR products purification, free sequence repetition in case of failure.
• Sample control quality: DNA normalization, multi-control quality steps during sequencing.
• Free universal primers  : a list of multiple universal primers provided by our lab.
• Online ordering system : Easy-to-use web page to enter your sequencing requests.
• Convenient data retrieval through secure web access : all results stored up to 5 years.

• High quality sequence: Read length up to 1 kb with a Q20 on ABI 3730XL equipment.
• Rapid turnaround time: Within 24 hours after sample receipt.
• 96 well samples submission: we do accept 4 Primers / plate or provide a mirror primers plate.
• Wide range of sample: plasmids, cosmids/fosmids/BAC, PCR products, single-strand DNA.
• Free services: Free PCR products purification, free sequence repetition in case of failure.
• Sample control quality: DNA normalization, multi-control quality steps during sequencing.
• Free universal primers  : a list of multiple universal primers provided by our lab.
• Online ordering system : Easy-to-use web page to enter your sequencing requests.
• Convenient data retrieval through secure web access : all results stored up to 5 years.

DNA Walking for Long Templates

DNA 1X or 2X Primer Walking: single strand ou double strand DNA walking, editing, sequence assembly up to 8 -10 kb. This service includes internal primers design and synthesis, successive single strand sequencing to progress along the DNA, manual sequence corrections, DNA assembly and the complete reconstruction of the sequence with the creation of a consensus sequence

Custom Project design : we can discuss all Specific project

Microsatellites, also referred as STR (Short Tandem Repeats) are small regions, widely dispersed on a given genome, that contain a repeated sequence of 2 to 9 bases (like GTGTGTGTGTGT). Due to polymerase slippage, the length of the motif can vary between individuals. Microsatellites are amplified by PCR with a couple of specific primers, whose one is fluorescent, and the size of the fragments obtained is analyzed on a capillary sequencer. Diploid organisms may be homozygote or heterozygote at a particular locus that leads to one or two peak profile on the respective electropherogram.

VNTR (Variable Number of Tandem Repeats) are minisatellites, that is, nucleotide patterns ranging from 10 to 100 bases typically repeated 5-50 times and located in certain regions of a given genome. Specific primers are used to PCR-amplify fragment that contain VNTRs. Their size of the fragments are measured on a capillary sequencer and by hence the number of repeats are deducted. Each variation of length represents an allele.

MLVA (Multiple Loci VNTR Analysis) use the length polymorphism of the VNTRs as a molecular method for the typing of organisms

MIRU (Mycobacterial Interspersed Repetitive Units) is a specific name for the MLVA analysis of the Mycobacterium tuberculosis complex. Similarly, the term SIRU is used for Staphylococcus aureus typing.

Dye / Fluorophore	Alternative Dye	Color
FAM	ATTO 488	BLUE
VIC / HEX / JOE	ATTO 532 / Yakima Yellow	GREEN
NED / TAMRA	ATTO 550	YELLOW
PET / ROX	ATTO 565	RED
LIZ (Standard Size)		ORANGE (for the internal ladder)