DNA Sequencing

Single run Sequencing

  • High quality sequence: Read length up to 1 kb with a Q20 on ABI 3730XL equipment.
  • Rapid turnaround time: Within 24 hours after sample receipt.
  • Flexible sample submission and pickup: samples submitted in different tubes or 8well-strips.
  • Wide range of sample: plasmids, cosmids/fosmids/BAC, PCR products, single-strand DNA.
  • Free services: Free PCR products purification, free sequence repetition in case of failure.
  • Sample control quality: DNA normalization, multi-control quality steps during sequencing.
  • Free universal primers  : a list of multiple universal primers provided by our lab.
  • Online ordering system : Easy-to-use web page to enter your sequencing requests.
  • Convenient data retrieval through secure web access : all results stored up to 5 years.

96 Well Single run Sequencing

  • High quality sequence: Read length up to 1 kb with a Q20 on ABI 3730XL equipment.
  • Rapid turnaround time: Within 24 hours after sample receipt.
  • 96 well samples submission: we do accept 4 Primers / plate or provide a mirror primers plate.
  • Wide range of sample: plasmids, cosmids/fosmids/BAC, PCR products, single-strand DNA.
  • Free services: Free PCR products purification, free sequence repetition in case of failure.
  • Sample control quality: DNA normalization, multi-control quality steps during sequencing.
  • Free universal primers  : a list of multiple universal primers provided by our lab.
  • Online ordering system : Easy-to-use web page to enter your sequencing requests.
  • Convenient data retrieval through secure web access : all results stored up to 5 years.

DNA Primer Walking

DNA Walking for Long Templates

DNA 1X or 2X Primer Walking: single strand ou double strand DNA walking, editing, sequence assembly up to 8 -10 kb. This service includes internal primers design and synthesis, successive single strand sequencing to progress along the DNA, manual sequence corrections, DNA assembly and the complete reconstruction of the sequence with the creation of a consensus sequence

Custom Project design : we can discuss all Specific project

How to use our Sanger sequencing services?

Click here for our web page ordering registration

Click here for our DNA Sequencing services Price list (lien vers Intranet- Liste de prix Sanger Sequencing)

Fragment analysis

Microsatellites, also referred as STR (Short Tandem Repeats) are small regions, widely dispersed on a given genome, that contain a repeated sequence of 2 to 9 bases (like GTGTGTGTGTGT). Due to polymerase slippage, the length of the motif can vary between individuals. Microsatellites are amplified by PCR with a couple of specific primers, whose one is fluorescent, and the size of the fragments obtained is analyzed on a capillary sequencer. Diploid organisms may be homozygote or heterozygote at a particular locus that leads to one or two peak profile on the respective electropherogram.

SNP & VNTR Analysis

VNTR (Variable Number of Tandem Repeats) are minisatellites, that is, nucleotide patterns ranging from 10 to 100 bases typically repeated 5-50 times and located in certain regions of a given genome. Specific primers are used to PCR-amplify fragment that contain VNTRs. Their size of the fragments are measured on a capillary sequencer and by hence the number of repeats are deducted. Each variation of length represents an allele.

MLVA (Multiple Loci VNTR Analysis) use the length polymorphism of the VNTRs as a molecular method for the typing of organisms

MIRU (Mycobacterial Interspersed Repetitive Units) is a specific name for the MLVA analysis of the Mycobacterium tuberculosis complex. Similarly, the term SIRU is used for Staphylococcus aureus typing.

Dye / FluorophoreAlternative DyeColor
FAMATTO 488BLUE
VIC / HEX / JOEATTO 532 / Yakima YellowGREEN
NED / TAMRAATTO 550YELLOW
PET / ROXATTO 565RED
LIZ (Standard Size)ORANGE (for the internal ladder)
2020

Gauthier M et al. Mol Ecol Resour. 2020 Jan;20(1):79-96 Biofidal has the pleasure to be co-author on this paper

2019
2018
2017

Adamo M et al. Can J Microbiol. 2017 Aug 9 : 1 – 10
Benamara RN. Thèse à l’Université Abou Bakr Belkaid de Tlemcen. 2017 April 29
Boulangé-Lecomte C et al. Comp Biochem Physiol C Toxicol Pharmacol. 2017 Sep 30. pii : S1532 – 0456 (17) 30166 – 7
Denet E et al. Parasitol Res. 2017 Nov ; 116 (11) : 3151 – 3162
Descours G et al. Antimicrob Agents Chemother. 2017 Jan 9. pii: AAC.02188-16
Desvars-Larrive A et al. PLoS One. 2017 Sep 8;12(9):e0184015
Dupont D et al. Mycopathologia. 2017
El-Habr EA et al. Acta Neuropathol. 2017 Apr;133(4):645-660
Eme D et al. Ecography. 2017 Fev 14
Espana B et al. Biopharm Drug Dispos. 2017 Jan; 38 (1): 20-32
Fougerolle S et al. Virology. 2017 May; 505: 210-217
Gasmi M. Thèse à l’Université des Frères Mentouri Constantine. 2017 Jun 29
Gelo-Pujic M and Amory A. 2017. Microorganisms and Methods for producing Vanillin
Gippet JMW et al. Urban Ecosyst. 2017; 20: 157
Goulois J et al. Ecol Evol. 2017; 00: 1–10
Hafirassou AZ. Thèse à l’université des Frères Mentouri Constantine. 2017 March 13
Keshavarz-Tohid V et al. Res Microbiol. 2017 Aug 26. pii : S0923 – 2508 (17) 30134 – 1
Khemiri H et al. African Zoology. 2017 Apr 03; 52 (1): 9–20
Koffi O et al. European Scientific J. 13 (30)
Malard F et al. Freshwater Biol. 2017 ; 62 : 1955 – 1970
Minard G et al. Parasit Vectors. 2017 Jun 2 ; 10 (1) : 276
Mouthon J and Forcellini M. BioInvasions Records. 2017; 6
Patot S et al. PLoS Pathog. 2017 Jan 6 ; 13 (1): e1006092
Pham Thi KL et al. Parasit Vectors. 2017 Nov 9;10(1):556
Pronost S et al. Transbound Emerg Dis. 2017 Dec;64(6):1884-1897
René-Martellet M et al. Parasit Vectors. 2017 Sep 7 ; 10 (1) : 416
Sánchez-Herrera et al. New Microbes New Infect. 2017 Apr 8 ; 19 : 96 – 116
Seifert B. et al. Myrmeco News. 2017 ; 27, 123–144
Sow F et al. Malar J. 2017 Feb 2;16(1):61

2016
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